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What is Cannabis Tissue Culture?

Cannabis tissue culture

Cannabis tissue culture leans more into the scientific side of cannabis plant biology rather than the general growing side.

Since the legalization of cannabis several years ago, various breakthroughs in cultivation and reproduction have cropped up, and all of them have brought significant advancement to the cannabis world. Plant tissue culture cannabis is one of the most innovative growing techniques. It rose to prominence between the 1950s and the 1960s. For instance, orchids benefited greatly from the plant tissue culture cannabis. Plant tissue culture cannabis became popular among farmers who used it to get the biggest yields. Moreover, plants grown in cannabis tissue culture have the added benefit of being disease-free, having increased vigor, and allowing you to produce a lot more in less space. You can also use it to boost the cannabis seed germination rate of older seeds, enable polyploidization, “reset” a sick parent plant, reset the internal clock of an autoflowering plant, and more.

What is plant tissue culture?

Plant tissue culture refers to extracting living tissue from a plant and growing that “explant” into a complete plant under ideal circumstances. Plant tissue culture cannabis may be more challenging with cannabis or hemp than other plant species. A significant number of tissue culture cuts with genetic similarities are the product of the cannabis tissue culture technique. It works flawlessly for small operations, but especially for large ones. Furthermore, the cannabis tissue culture protocol involves clonal propagation of the plant in a sterile environment. The method extracts secondary compounds from plants, most of which are cannabinoids.

Additionally, another term used in plant tissue culture is cannabis. Micropropagation is a technique for producing large numbers of new plants from fewer parent plants, resulting in cuts with consistent, predictable characteristics. Conversely, the cannabis industry misses a lot of this technique’s advantages because this species of plant is tough to micropropagate. This process has been highly successful due to the plant’s cells’ ability to regenerate into a completely new plant.

Phases of plant tissue culture cannabis

The plant tissue culture method has five distinct stages that aid in obtaining the required sample.

  1. Determine which plant you want to use.
  2. Initiation – placing or transferring mature plants and materials into a sterile area or a vitro environment.
  3. Micropropagation – this involves removing plants from the vitro environment and chopping them into smaller pieces, also known as the x-plant, which you store in a separate location. The cannabis micropropagation process increases their number.
  4. Rooting – is the process of stopping the x-plant from multiplying and then transferring it to a rooting medium.
  5. Acclimatization – this phase marks the official end of the cannabis micropropagation process and the return to the real world. However, you need a few more pieces of equipment to sterilize or disinfect the medium used to grow the samples.

How does plant tissue culture cannabis work?

The charm of cannabis tissue culture is that, unlike traditional techniques, it allows cultivators to retain a living specimen almost indefinitely with minimal effort and space. Small pieces of plant tissue, even a few cells, taken from cannabis specimens can generate hundreds of identical cuts. Every tissue culture begins with a small cutting from a sample. Trim the tissue sample before entering the critical sterilization process. Once the plant tissue has been cleaned, it is placed in a dense nutrient culture, generally, an agar gel comprising a carefully crafted nutrient, hormone, and sugar mixture. The sample is under the control of the tissue culture. Different hormone combinations will cause the tiny culture to go through different phases of development.

Tissue culture cannabis

Additionally, the culture can remain in an indeterminate purgatory state until the grower decides to work with the sample again. A cultivator can stimulate growth, root development, and multiplication by introducing new hormones and nutrient-rich growing solutions. When the crop has grown large enough to multiply, it is ready to be trimmed into hundreds of distinct but precisely cut individuals.

Benefits of cannabis tissue culture

For various reasons, cultivating crops using cannabis tissue cultures is preferable to using seeds or cuttings. Among them are the following:

  1. It is possible to store desirable genetic materials for a longer period which breeders can take advantage of.
  2. Because of its stable genetics, it allows for the cannabis tissue culture cloning of plants with very adorable attributes.
  3. It outperforms traditional cuttings in terms of disease resistance.
  4. It makes room for more tissue culture cuts to be stored.
  5. Small tissue samples can be used to create identical cuts.

Drawbacks of cannabis tissue culture

Cannabis tissue culture, despite its many attractive characteristics, has some challenges and drawbacks. Among them are the following:

  1. Growers must purchase specialized equipment for the cannabis tissue culture cloning and storing operation before they can start.
  2. If conventional cuttings take only two weeks to mature, cuts require nearly one month to fully mature and are ready to be transplanted.
  3. To use this technique, you must be in a controlled setting.

Where should you set up your “laboratory” for tissue culture?

Controlling the growing environment is required for tissue culture to work. You’ll get a great outcome if you keep the grow tent temperature, humidity, light intensity, light period/photoperiod, and nutrients for cannabis at the proper levels. Aim for:

  • A place where there is little foot traffic.
  • Temperature stability and temperature control around 21C or 70F.
  • Water and a drain are available.
  • Simple to clean.
  • Air is free of dust, mold, spores, and chemicals.

Plant tissue culture cannabis is an advanced cultivation procedure that can benefit all cannabis growers who want to increase yield and harvest performance. This technique, however, is not suitable for those who do not like to wait too long before enjoying their favorite crops. There may be numerous benefits to using plant tissue culture cannabis as a planting technique. If you plan to grow your crops, sticking to what you know is safe, but it’s never a bad idea to try highly creative cannabis cultivation methods. Tissue culture cannabis is simply an alternative you can pursue if you have the opportunity.

Picture of Ed Rushford

Ed Rushford

Ed Rushford’s impact on cannabis growing is undeniable. Though he tends to focus primarily on 2 areas, plant training techniques and dealing with disease, pests, and other problems, he has offered many insights into how cannabis plants live and grow. That’s not to say that Ed is unfamiliar with the complete life cycle of cannabis, from seed to harvest, but he uses his widespread knowledge to hone in on the minutia and niche areas of growing cannabis. Ed’s goal is to spread knowledge and allow for everyone to become better growers. About this Author

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